Rabbit IgG anti-Dog IgG (F(ab)2)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Dog IgG (F(ab)2)-HRPO, MinX none
• Biozol Catalog Number: ROC-604-4304
• Supplier Catalog Number: 604-4304
• Alternative Catalog Number: ROC-604-4304
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Canine
• Immunogen: Dog IgG F(ab)2 fragment
• Conjugation: HRPO
• Alternative Names: rabbit anti-dog IgG F(ab)2 Antibody Peroxidase conjugation, rabbit anti-Dog IgG Fab2 fragment HRP conjugated Antibody
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Dog
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:20,000
• Application Notes: Anti-Dog IgG F(ab)2 Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot co

Images

ELISA results of purified Rabbit Anti-Dog IgG F(ab)2 Antibody tested against purified Dog IgG F(ab)2. Each well was coated in duplicate with 1.0 µg of Dog IgG F(ab)2 (red line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.