Rabbit IgG anti-Goat IgG (F(ab)2)-HRPO, MinX none

Artikelnummer: ROC-605-4304
Artikelname: Rabbit IgG anti-Goat IgG (F(ab)2)-HRPO, MinX none
Artikelnummer: ROC-605-4304
Hersteller Artikelnummer: 605-4304
Alternativnummer: ROC-605-4304
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Goat
Immunogen: Goat IgG F(ab)2 fragment
Konjugation: HRPO
Alternative Synonym: rabbit anti-goat IgG F(ab)2 HRP conjugated, rabbit anti-goat IgG F(ab)2 peroxidase conjugated, rabbit anti-goat IgG Fab2 HRP conjugated, rabbit anti-goat IgG Fab2 peroxidase conjugated
Format: IgG
Spezifität: IgG (F(ab')2)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Goat
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Anwendungsbeschreibung: Anti-Goat IgG F(ab)2 Peroxidase Antibody has been tested by ELISA and is assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at ro
ELISA results of purified Rabbit Anti-Goat IgG F(ab)2 Antibody tested against purified Goat IgG F(ab)2 (green line). Each well was coated in duplicate with 1.0 µg of Goat IgG F(ab)2. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.