Rabbit IgG anti-Goat IgG (F(ab)2)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Goat IgG (F(ab)2)-HRPO, MinX none
• Biozol Catalog Number: ROC-605-4304
• Supplier Catalog Number: 605-4304
• Alternative Catalog Number: ROC-605-4304
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Goat
• Immunogen: Goat IgG F(ab)2 fragment
• Conjugation: HRPO
• Alternative Names: rabbit anti-goat IgG F(ab)2 HRP conjugated, rabbit anti-goat IgG F(ab)2 peroxidase conjugated, rabbit anti-goat IgG Fab2 HRP conjugated, rabbit anti-goat IgG Fab2 peroxidase conjugated
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Goat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: Anti-Goat IgG F(ab)2 Peroxidase Antibody has been tested by ELISA and is assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at ro

Images

ELISA results of purified Rabbit Anti-Goat IgG F(ab)2 Antibody tested against purified Goat IgG F(ab)2 (green line). Each well was coated in duplicate with 1.0 µg of Goat IgG F(ab)2. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.