Goat IgG anti-Horse IgG (F(ab)2)-HRPO, MinX none

Artikelnummer: ROC-608-1304
Artikelname: Goat IgG anti-Horse IgG (F(ab)2)-HRPO, MinX none
Artikelnummer: ROC-608-1304
Hersteller Artikelnummer: 608-1304
Alternativnummer: ROC-608-1304
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Equine
Immunogen: Anti-Horse IgG was produced by repeated immunization with horse IgG F(ab)2 fragment in goat.
Konjugation: HRPO
Alternative Synonym: Horse IgG F(ab)2 Antibody, Gt-a-Horse F(ab)2 Peroxidase conjugated, Horse F(ab)2 Peroxidase Antibody in goat, Goat anti-horse F(ab)2 peroxidase conjugated Secondary Antibody
Format: IgG
Spezifität: IgG (F(ab')2)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Horse
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:50,000 - 1:150,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
Anwendungsbeschreibung: Anti-Horse IgG F(ab)2 peroxidase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzyma
ELISA results of purified Polyclonal Goat Anti-Horse IgG F(ab)2 Antibody Peroxidase Conjugated tested against Horse IgG F(ab)2 (red) and Horse IgG (green). Each well was coated in duplicate with 1.0µg of antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).