Goat IgG anti-Horse IgG (F(ab)2)-HRPO, MinX none

Product Overview

• Article Name: Goat IgG anti-Horse IgG (F(ab)2)-HRPO, MinX none
• Biozol Catalog Number: ROC-608-1304
• Supplier Catalog Number: 608-1304
• Alternative Catalog Number: ROC-608-1304
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Equine
• Immunogen: Anti-Horse IgG was produced by repeated immunization with horse IgG F(ab)2 fragment in goat.
• Conjugation: HRPO
• Alternative Names: Horse IgG F(ab)2 Antibody, Gt-a-Horse F(ab)2 Peroxidase conjugated, Horse F(ab)2 Peroxidase Antibody in goat, Goat anti-horse F(ab)2 peroxidase conjugated Secondary Antibody
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Horse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:50,000 - 1:150,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Horse IgG F(ab)2 peroxidase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzyma

Images

ELISA results of purified Polyclonal Goat Anti-Horse IgG F(ab)2 Antibody Peroxidase Conjugated tested against Horse IgG F(ab)2 (red) and Horse IgG (green). Each well was coated in duplicate with 1.0µg of antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).