Goat IgG anti-Human IgG (Fc)-Biotin, MinX Ms, Polyclonal

Produktübersicht

• Artikelname: Goat IgG anti-Human IgG (Fc)-Biotin, MinX Ms, Polyclonal
• Artikelnummer: ROC-609-106-017
• Hersteller Artikelnummer: 609-106-017
• Alternativnummer: ROC-609-106-017
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Human
• Immunogen: Human IgG F(c) fragment
• Konjugation: Biotin
• Alternative Synonym: Goat Anti Human IgG Fc Biotin Conjugation Antibody, Goat Anti-Human IgG F(c) Antibody Biotin Conjugated, Goat Anti Human IgG Fc Fragment Antibody Biotin Conjugate
• Format: IgG
• Spezifität: IgG (Fc)
• Minimale Kreuzreaktivität (MinX): Mouse

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Human
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:194,000 - 1:294,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: Anti-Human IgG F(c) Biotin Conjugated has been tested by ELISA and is designed for Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods.

Bilder

ELISA results of purified Goat anti-Human IgG F(c) Antibody Biotin conjugated tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n 009-0103). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated (p/n S000-03) and TMB substrate p/n TMBE-1000.