Goat IgG anti-Human IgG (Fc)-Biotin, MinX Ms, Polyclonal

Catalog Number: ROC-609-106-017
Article Name: Goat IgG anti-Human IgG (Fc)-Biotin, MinX Ms, Polyclonal
Biozol Catalog Number: ROC-609-106-017
Supplier Catalog Number: 609-106-017
Alternative Catalog Number: ROC-609-106-017
Manufacturer: Rockland Immunochemicals
Host: Goat
Category: Antikörper
Application: ELISA,IHC,WB
Species Reactivity: Human
Immunogen: Human IgG F(c) fragment
Conjugation: Biotin
Alternative Names: Goat Anti Human IgG Fc Biotin Conjugation Antibody, Goat Anti-Human IgG F(c) Antibody Biotin Conjugated, Goat Anti Human IgG Fc Fragment Antibody Biotin Conjugate
Format: IgG
Target Specificity: IgG (Fc)
Cross-Adsorption (MinX): Mouse
Clonality: Polyclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Lyophilized
Target: Human
Antibody Type: Secondary Antibody
Application Dilute: ELISA: 1:194,000 - 1:294,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
Application Notes: Anti-Human IgG F(c) Biotin Conjugated has been tested by ELISA and is designed for Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods.
ELISA results of purified Goat anti-Human IgG F(c) Antibody Biotin conjugated tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n 009-0103). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated (p/n S000-03) and TMB substrate p/n TMBE-1000.