Anti-Human IgG (H&L) Biotin conjugated Antibody has been tested by ELISA and western blot and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods
Western Blot of Goat anti-Human IgG Pre-Adsorbed Biotin Conjugated Secondary Antibody. Lane 1: Human IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Biotin conjugated goat anti-Human IgG 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Human IgG. Other band(s): None.
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