Goat IgG anti-Human IgG (H+L)-Biotin, MinX Bo,Ck,Go,Gp,Hm,Ho,Ms,Rb,Rt,Sh, Polyclonal

Product Overview

• Article Name: Goat IgG anti-Human IgG (H+L)-Biotin, MinX Bo,Ck,Go,Gp,Hm,Ho,Ms,Rb,Rt,Sh, Polyclonal
• Biozol Catalog Number: ROC-609-106-123
• Supplier Catalog Number: 609-106-123
• Alternative Catalog Number: ROC-609-106-123
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: Biotin
• Alternative Names: Anti Human IgG Secondary Antibody, Biotin Anti Human IgG, Conjugated Secondary Antibodies
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Mouse,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Human IgG (H&L) Biotin conjugated Antibody has been tested by ELISA and western blot and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods

Images

Western Blot of Goat anti-Human IgG Pre-Adsorbed Biotin Conjugated Secondary Antibody. Lane 1: Human IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Biotin conjugated goat anti-Human IgG 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Human IgG. Other band(s): None.