Mouse IgG anti-Human IgG (H+L)-unconj., MinX none

Produktübersicht

• Artikelname: Mouse IgG anti-Human IgG (H+L)-unconj., MinX none
• Artikelnummer: ROC-609-301-002
• Hersteller Artikelnummer: 609-301-002
• Alternativnummer: ROC-609-301-002
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Human
• Immunogen: Human IgG whole molecule
• Konjugation: Unconjugated
• Alternative Synonym: Mouse Anti-Human IgG Antibody, Mouse Anti Human IgG Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:5,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:500 - 1:5,000
• Anwendungsbeschreibung: Mouse Anti-Human IgG (H&L) has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection sho

Bilder

Western Blot of Human IgG Antibody. M: 3 µL Opal Prestained Ladder (MB-210-0500). Lane 1: Human IgG (p/n 009-0102). Load: 50 ng per lane. Primary antibody: Human IgG Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase conjugated mouse secondary antibody at 1:40,000 for 30 minutes at RT. Block: (p/n MB-070) for 30 minutes at RT. Predicted/Observed size: 55 kDa, 25 kDa for Human IgG.