Mouse IgG anti-Human IgG (H+L)-unconj., MinX none

Product Overview

• Article Name: Mouse IgG anti-Human IgG (H+L)-unconj., MinX none
• Biozol Catalog Number: ROC-609-301-002
• Supplier Catalog Number: 609-301-002
• Alternative Catalog Number: ROC-609-301-002
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Mouse Anti-Human IgG Antibody, Mouse Anti Human IgG Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:500 - 1:5,000
• Application Notes: Mouse Anti-Human IgG (H&L) has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection sho

Images

Western Blot of Human IgG Antibody. M: 3 µL Opal Prestained Ladder (MB-210-0500). Lane 1: Human IgG (p/n 009-0102). Load: 50 ng per lane. Primary antibody: Human IgG Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase conjugated mouse secondary antibody at 1:40,000 for 30 minutes at RT. Block: (p/n MB-070) for 30 minutes at RT. Predicted/Observed size: 55 kDa, 25 kDa for Human IgG.