Mouse IgG anti-Human IgG (H+L)-FITC, MinX none

Produktübersicht

• Artikelname: Mouse IgG anti-Human IgG (H+L)-FITC, MinX none
• Artikelnummer: ROC-609-302-002
• Hersteller Artikelnummer: 609-302-002
• Alternativnummer: ROC-609-302-002
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FLISA,FACS,IF
• Spezies Reaktivität: Human
• Immunogen: Human IgG whole molecule
• Konjugation: FITC
• Alternative Synonym: mouse anti-Human IgG fluorescein conjugated Antibody, mouse anti-Human IgG Antibody FITC conjugation
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Human
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: Mouse Anti-Human IgG (H&L) Antibody Fluorescein Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiple

Bilder

Dot Blot showing the detection of Human IgG. A three-fold serial dilution of Human IgG starting at 200ng was spotted onto 0.45 µm nitrocellulose. After blocking in 5% Blotto (p/n B501-0500) 1 Hour at 20C, Anti-Human IgG (H&L) (MOUSE) Antibody Fluorescein Conjugated (p/n 609-302-002) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc 4000 MP.