Mouse IgG anti-Human IgG (H+L)-FITC, MinX none

Product Overview

• Article Name: Mouse IgG anti-Human IgG (H+L)-FITC, MinX none
• Biozol Catalog Number: ROC-609-302-002
• Supplier Catalog Number: 609-302-002
• Alternative Catalog Number: ROC-609-302-002
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: FLISA,FACS,IF
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: FITC
• Alternative Names: mouse anti-Human IgG fluorescein conjugated Antibody, mouse anti-Human IgG Antibody FITC conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: Mouse Anti-Human IgG (H&L) Antibody Fluorescein Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiple

Images

Dot Blot showing the detection of Human IgG. A three-fold serial dilution of Human IgG starting at 200ng was spotted onto 0.45 µm nitrocellulose. After blocking in 5% Blotto (p/n B501-0500) 1 Hour at 20C, Anti-Human IgG (H&L) (MOUSE) Antibody Fluorescein Conjugated (p/n 609-302-002) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc 4000 MP.