Anti-Human IgG F(c) fragment has been tested by western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be o
Western Blot of Rabbit anti-Human Fc antibody. Lane 1: Human Fc (p/n 009-0103). Lane 2: none. Load: 100 ng per lane. Primary antibody: Human Fc antibody at 1:1,000 for overnight at 4C. Secondary antibody: DyLight(TM) 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Human Fc. Other band(s): Human Fc splice variants and isoforms.
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