Rabbit IgG anti-Human IgG (Fc)-unconj., MinX none

Product Overview

• Article Name: Rabbit IgG anti-Human IgG (Fc)-unconj., MinX none
• Biozol Catalog Number: ROC-609-4103
• Supplier Catalog Number: 609-4103
• Alternative Catalog Number: ROC-609-4103
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Anti-Human IgG F(c) fragment was produced by repeated immunization with Human IgG F(c) fragment in rabbit.
• Conjugation: Unconjugated
• Alternative Names: Human IgG F(c) Antibody, Human IgG Fc Antibody, Rb-a-Human IgG Fc, Human IgG F(c) Antibody in Rabbit, rabbit anti-human IgG F(c) Secondary Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 5.014 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:26,000-1:46,000, IHC: 1:1,000 - 1:6,000, WB: 1:3,000 - 1:30,000
• Application Notes: Anti-Human IgG F(c) fragment has been tested by western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be o

Images

Western Blot of Rabbit anti-Human Fc antibody. Lane 1: Human Fc (p/n 009-0103). Lane 2: none. Load: 100 ng per lane. Primary antibody: Human Fc antibody at 1:1,000 for overnight at 4C. Secondary antibody: DyLight(TM) 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Human Fc. Other band(s): Human Fc splice variants and isoforms.