Rabbit IgG anti-Human IgG (F(ab)2)-HRPO, MinX Ms, Polyclonal

Produktübersicht

• Artikelname: Rabbit IgG anti-Human IgG (F(ab)2)-HRPO, MinX Ms, Polyclonal
• Artikelnummer: ROC-609-4318
• Hersteller Artikelnummer: 609-4318
• Alternativnummer: ROC-609-4318
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Human
• Immunogen: Human IgG F(ab)2 fragment
• Konjugation: HRPO
• Alternative Synonym: rabbit Anti-Human IgG Fab2 Antibody peroxidase Conjugated, Rabbit Anti-Human IgG F(ab)2 Antibody peroxidase Conjugated, Rabbit Anti-Human IgG Fab2 Fragment Antibody HRP Conjugated
• Format: IgG
• Spezifität: IgG (F(ab')2)
• Minimale Kreuzreaktivität (MinX): Mouse

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Human
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Human IgG F(ab)2 Peroxidase Antibody has been tested by ELISA and is assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature

Bilder

ELISA results of purified Rabbit Anti-Human IgG F(ab)2 Peroxidase Conjugated Antibody tested against purified Human IgG F(ab)2. Each well was coated in duplicate with 1.0 µg of Human IgG F(ab)2 (blue line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.