Rabbit IgG anti-Human IgG (F(ab)2)-HRPO, MinX Ms, Polyclonal

Product Overview

• Article Name: Rabbit IgG anti-Human IgG (F(ab)2)-HRPO, MinX Ms, Polyclonal
• Biozol Catalog Number: ROC-609-4318
• Supplier Catalog Number: 609-4318
• Alternative Catalog Number: ROC-609-4318
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG F(ab)2 fragment
• Conjugation: HRPO
• Alternative Names: rabbit Anti-Human IgG Fab2 Antibody peroxidase Conjugated, Rabbit Anti-Human IgG F(ab)2 Antibody peroxidase Conjugated, Rabbit Anti-Human IgG Fab2 Fragment Antibody HRP Conjugated
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): Mouse

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: Anti-Human IgG F(ab)2 Peroxidase Antibody has been tested by ELISA and is assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature

Images

ELISA results of purified Rabbit Anti-Human IgG F(ab)2 Peroxidase Conjugated Antibody tested against purified Human IgG F(ab)2. Each well was coated in duplicate with 1.0 µg of Human IgG F(ab)2 (blue line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.