Anti-Human IgG (H&L) Biotin has been tested by western blot and ELISA and has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic
Western Blot of Rabbit anti-Human IgG (H&L) Antibody Biotin Conjugated Lane 1: Human IgG Load: 50 ng per lane Primary antibody: Human IgG (H&L) Antibody Biotin Conjugated at 1:1000 for 60 min RT Secondary antibody: HRP Conjugated Streptavidin at 1:40,000 for 30 min at RT Block: MB-070 for 30 min at RT
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