Rabbit IgG anti-Human IgG (H+L)-Biotin, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Human IgG (H+L)-Biotin, MinX none
• Biozol Catalog Number: ROC-609-4602
• Supplier Catalog Number: 609-4602
• Alternative Catalog Number: ROC-609-4602
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: Biotin
• Alternative Names: Rabbit Anti Human IgG Biotin Conjugated Antibody, Rabbit Anti-Human IgG Antibody Biotin Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Human IgG (H&L) Biotin has been tested by western blot and ELISA and has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic

Images

Western Blot of Rabbit anti-Human IgG (H&L) Antibody Biotin Conjugated Lane 1: Human IgG Load: 50 ng per lane Primary antibody: Human IgG (H&L) Antibody Biotin Conjugated at 1:1000 for 60 min RT Secondary antibody: HRP Conjugated Streptavidin at 1:40,000 for 30 min at RT Block: MB-070 for 30 min at RT