Sheep IgG anti-Human IgG (H+L)-HRPO, MinX none

Produktübersicht

• Artikelname: Sheep IgG anti-Human IgG (H+L)-HRPO, MinX none
• Artikelnummer: ROC-609-6302
• Hersteller Artikelnummer: 609-6302
• Alternativnummer: ROC-609-6302
• Hersteller: Rockland Immunochemicals
• Wirt: Sheep
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Human
• Immunogen: Human IgG whole molecule
• Konjugation: HRPO
• Alternative Synonym: sheep anti-Human IgG Antibody peroxidase Conjugation, sheep anti-Human IgG HRP Conjugated Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Human
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:150,000 - 1:250,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Human IgG (H&L) Peroxidase has been tested by ELISA and is assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room tempe

Bilder

ELISA results of purified Sheep Anti-Human IgG Peroxidase Conjugated Antibody tested against purified Human IgG. Each well was coated in duplicate with 1.0 µg of Human IgG (red line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.