Sheep IgG anti-Human IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Sheep IgG anti-Human IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-609-6302
• Supplier Catalog Number: 609-6302
• Alternative Catalog Number: ROC-609-6302
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: HRPO
• Alternative Names: sheep anti-Human IgG Antibody peroxidase Conjugation, sheep anti-Human IgG HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:150,000 - 1:250,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: Anti-Human IgG (H&L) Peroxidase has been tested by ELISA and is assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room tempe

Images

ELISA results of purified Sheep Anti-Human IgG Peroxidase Conjugated Antibody tested against purified Human IgG. Each well was coated in duplicate with 1.0 µg of Human IgG (red line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.