Sheep IgG anti-Human IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Artikelnummer: ROC-609-6502
Artikelname: Sheep IgG anti-Human IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
Artikelnummer: ROC-609-6502
Hersteller Artikelnummer: 609-6502
Alternativnummer: ROC-609-6502
Hersteller: Rockland Immunochemicals
Wirt: Sheep
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Human
Immunogen: Human IgG whole molecule
Konjugation: Alk. Phos.
Alternative Synonym: sheep anti-Human IgG Antibody alkaline phosphatase Conjugation, sheep anti-Human IgG alk phos Conjugated Antibody
Format: IgG
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Anwendungsbeschreibung: Anti-HUMAN IgG (H&L) Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody b
Dot Blot of Sheep anti-Human IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Human IgG. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Sheep anti-Human IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Visualized using NBT-100 Alkaline Phosphatase Substrate for 30 seconds at RT.