Sheep IgG anti-Human IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Sheep IgG anti-Human IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-609-6502
• Supplier Catalog Number: 609-6502
• Alternative Catalog Number: ROC-609-6502
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: Alk. Phos.
• Alternative Names: sheep anti-Human IgG Antibody alkaline phosphatase Conjugation, sheep anti-Human IgG alk phos Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-HUMAN IgG (H&L) Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody b

Images

Dot Blot of Sheep anti-Human IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Human IgG. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Sheep anti-Human IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Visualized using NBT-100 Alkaline Phosphatase Substrate for 30 seconds at RT.