Goat IgG anti-Mouse IgG3 (Fc)-unconj., MinX none

Produktübersicht

• Artikelname: Goat IgG anti-Mouse IgG3 (Fc)-unconj., MinX none
• Artikelnummer: ROC-610-101-043
• Hersteller Artikelnummer: 610-101-043
• Alternativnummer: ROC-610-101-043
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgG3 heavy chain
• Konjugation: Unconjugated
• Alternative Synonym: goat anti-MOUSE IgG3 Antibody, goat anti-MOUSE IgG3 (gamma 3) Antibody
• Format: IgG
• Spezifität: IgG3 (Fc)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:40,000 - 1:200,000, IHC: 1:2,000 - 1:10,000, WB: 1:4,000 - 1:20,000
• Anwendungsbeschreibung: Anti-Mouse IgG3 secondary antibody has been tested by ELISA and is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG3 antibody based assays. Mouse IgG3 secondary antibody is available in a variety of formats.

Bilder

ELISA Results of Goat Anti-Mouse IgG3 (Gamma 3 chain) Antibody Minimum Cross Reactivity to Bovine, Human, and Rabbit Serum Proteins tested against purified Mouse IgG3 mx3. Each well was coated in duplicate with 1.0 µg of Mouse IgG3 (p/n 010-0143). The working dilution is 1:90,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Donkey Anti-Goat IgG HRP (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).