Goat IgG anti-Mouse IgG3 (Fc)-unconj., MinX none

Product Overview

• Article Name: Goat IgG anti-Mouse IgG3 (Fc)-unconj., MinX none
• Biozol Catalog Number: ROC-610-101-043
• Supplier Catalog Number: 610-101-043
• Alternative Catalog Number: ROC-610-101-043
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG3 heavy chain
• Conjugation: Unconjugated
• Alternative Names: goat anti-MOUSE IgG3 Antibody, goat anti-MOUSE IgG3 (gamma 3) Antibody
• Format: IgG
• Target Specificity: IgG3 (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:40,000 - 1:200,000, IHC: 1:2,000 - 1:10,000, WB: 1:4,000 - 1:20,000
• Application Notes: Anti-Mouse IgG3 secondary antibody has been tested by ELISA and is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG3 antibody based assays. Mouse IgG3 secondary antibody is available in a variety of formats.

Images

ELISA Results of Goat Anti-Mouse IgG3 (Gamma 3 chain) Antibody Minimum Cross Reactivity to Bovine, Human, and Rabbit Serum Proteins tested against purified Mouse IgG3 mx3. Each well was coated in duplicate with 1.0 µg of Mouse IgG3 (p/n 010-0143). The working dilution is 1:90,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Donkey Anti-Goat IgG HRP (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).