Goat IgG anti-Mouse IgG1 (Fc)-HRPO, MinX none

Artikelnummer: ROC-610-103-040
Artikelname: Goat IgG anti-Mouse IgG1 (Fc)-HRPO, MinX none
Artikelnummer: ROC-610-103-040
Hersteller Artikelnummer: 610-103-040
Alternativnummer: ROC-610-103-040
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Mouse
Immunogen: Mouse IgG1 heavy chain
Konjugation: HRPO
Alternative Synonym: Goat anti-mouse IgG1 antibody peroxidase conjugation, goat anti-mouse IgG1 (gamma 1) HRP conjugated antibody
Format: IgG
Spezifität: IgG1 (Fc)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Mouse
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000
Anwendungsbeschreibung: Anti-Mouse IgG1 Peroxidase Antibody has been tested by ELISA and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
ELISA Results of Goat Anti-Mouse IgG1 mx3 Antibody Peroxidase Conjugated tested against purified Mouse IgG1 mx3. Each well was coated in duplicate with 1.0 µg of Mouse IgG1 (p/n 010-0140). The working dilution of Mouse IgG1 mx3 HRP is 1:100,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).