Goat IgG anti-Mouse IgG1 (Fc)-HRPO, MinX none

Product Overview

• Article Name: Goat IgG anti-Mouse IgG1 (Fc)-HRPO, MinX none
• Biozol Catalog Number: ROC-610-103-040
• Supplier Catalog Number: 610-103-040
• Alternative Catalog Number: ROC-610-103-040
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG1 heavy chain
• Conjugation: HRPO
• Alternative Names: Goat anti-mouse IgG1 antibody peroxidase conjugation, goat anti-mouse IgG1 (gamma 1) HRP conjugated antibody
• Format: IgG
• Target Specificity: IgG1 (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000
• Application Notes: Anti-Mouse IgG1 Peroxidase Antibody has been tested by ELISA and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.

Images

ELISA Results of Goat Anti-Mouse IgG1 mx3 Antibody Peroxidase Conjugated tested against purified Mouse IgG1 mx3. Each well was coated in duplicate with 1.0 µg of Mouse IgG1 (p/n 010-0140). The working dilution of Mouse IgG1 mx3 HRP is 1:100,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).