Anti-Mouse IgG Biotin Antibody has been tested by ELISA and is ideal for western blot and are available in a variety of formats and conjugate types.
ELISA results of purified Goat Anti-Mouse IgG Biotin Conjugated Antibody MinX 10 tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (red line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP, and TMB-1000 substrate.
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