Goat IgG anti-Mouse IgG (H+L)-Biotin, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, Polyclonal

Product Overview

• Article Name: Goat IgG anti-Mouse IgG (H+L)-Biotin, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, Polyclonal
• Biozol Catalog Number: ROC-610-106-121
• Supplier Catalog Number: 610-106-121
• Alternative Catalog Number: ROC-610-106-121
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG whole molecule
• Conjugation: Biotin
• Alternative Names: Goat Anti-Mouse IgG Secondary Antibody biotin Conjugation, Goat Anti-Mouse IgG Antibody biotin Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG biotin conjugated secondary antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:100,000 - 1:800,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Mouse IgG Biotin Antibody has been tested by ELISA and is ideal for western blot and are available in a variety of formats and conjugate types.

Images

ELISA results of purified Goat Anti-Mouse IgG Biotin Conjugated Antibody MinX 10 tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (red line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP, and TMB-1000 substrate.