Goat IgG anti-Mouse IgM (µ)-unconj., MinX none

Artikelnummer: ROC-610-1107
Artikelname: Goat IgG anti-Mouse IgM (µ)-unconj., MinX none
Artikelnummer: ROC-610-1107
Hersteller Artikelnummer: 610-1107
Alternativnummer: ROC-610-1107
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Mouse
Immunogen: Mouse IgM whole molecule
Konjugation: Unconjugated
Alternative Synonym: goat anti-Mouse IgM (mu chain) Antibody, Gt-a-Mouse (mu chain) antibody, Mouse IgM (mu chain) Antibody in goat, Goat anti-mouse IgM Secondary Antibody
Format: IgG
Spezifität: IgM (µ)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 2.19 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Mouse
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:20,400 - 1:40,400, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Anwendungsbeschreibung: Anti-Mouse IgM (mu chain) has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.
Dot Blot of Mouse IgM (mu chain) (GOAT) Antibody. Lane 1A: Mouse IgM (100 ng). Lane 1B: Mouse IgG (100 ng). Lanes 2-5: serial 3-fold dilution. Primary Antibody: Mouse IgM (mu chain) Antibody at 1 µg/mL at RT for 60 minutes. Secondary Antibody: Donkey Anti-Goat IgG Antibody HRP Conjd (p/n 605-703-125) at 1,40,000 dilution of 1 mg/mL at RT for 30 minutes. Block: MB-070 at RT for 30 min.