Goat IgG anti-Mouse IgM (µ)-unconj., MinX none

Product Overview

• Article Name: Goat IgG anti-Mouse IgM (µ)-unconj., MinX none
• Biozol Catalog Number: ROC-610-1107
• Supplier Catalog Number: 610-1107
• Alternative Catalog Number: ROC-610-1107
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgM whole molecule
• Conjugation: Unconjugated
• Alternative Names: goat anti-Mouse IgM (mu chain) Antibody, Gt-a-Mouse (mu chain) antibody, Mouse IgM (mu chain) Antibody in goat, Goat anti-mouse IgM Secondary Antibody
• Format: IgG
• Target Specificity: IgM (µ)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.19 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,400 - 1:40,400, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Mouse IgM (mu chain) has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.

Images

Dot Blot of Mouse IgM (mu chain) (GOAT) Antibody. Lane 1A: Mouse IgM (100 ng). Lane 1B: Mouse IgG (100 ng). Lanes 2-5: serial 3-fold dilution. Primary Antibody: Mouse IgM (mu chain) Antibody at 1 µg/mL at RT for 60 minutes. Secondary Antibody: Donkey Anti-Goat IgG Antibody HRP Conjd (p/n 605-703-125) at 1,40,000 dilution of 1 mg/mL at RT for 30 minutes. Block: MB-070 at RT for 30 min.