Goat IgG anti-Mouse IgG (Fc)-unconj., MinX Bo,Ho,Hu, Polyclonal , Unconjugated

Artikelnummer: ROC-610-1122
Artikelname: Goat IgG anti-Mouse IgG (Fc)-unconj., MinX Bo,Ho,Hu, Polyclonal , Unconjugated
Artikelnummer: ROC-610-1122
Hersteller Artikelnummer: 610-1122
Alternativnummer: ROC-610-1122
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Mouse
Immunogen: Mouse IgG F(c) fragment
Konjugation: Unconjugated
Alternative Synonym: Immunoglobulin, Mouse IgG, Goat Anti-Mouse IgG F(c) Antibody, Goat Anti-Mouse IgG Fc Antibody, Goat Anti-Mouse IgG Fc Fragment Antibody
Format: IgG
Spezifität: IgG (Fc)
Minimale Kreuzreaktivität (MinX): Bovine,Equine,Human
Klonalität: Polyclonal
Konzentration: 1.20 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Mouse
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:450,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:50,000
Anwendungsbeschreibung: Anti-Mouse IgG F(c) antibody has been tested by ELISA and is suitable for ELISA, Western Blot and Immunohistochemistry applications. Antibody should be optimized by end user for specific reactive conditions.
ELISA Results of purified Goat Anti-Mouse IgG F(c) Min X Bovine Horse and Human Serums. Each well was coated in duplicate with 1.0 µg of Mouse IgG (010-0102). The working dilution is 27300. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Dky Anti- Goat IgG HRP conjugated (p/n 605-703-125) and TMB substrate (p/n TMBE-1000).