Goat IgG anti-Mouse IgG (Fc)-unconj., MinX Bo,Ho,Hu, Polyclonal , Unconjugated

Product Overview

• Article Name: Goat IgG anti-Mouse IgG (Fc)-unconj., MinX Bo,Ho,Hu, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-610-1122
• Supplier Catalog Number: 610-1122
• Alternative Catalog Number: ROC-610-1122
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG F(c) fragment
• Conjugation: Unconjugated
• Alternative Names: Immunoglobulin, Mouse IgG, Goat Anti-Mouse IgG F(c) Antibody, Goat Anti-Mouse IgG Fc Antibody, Goat Anti-Mouse IgG Fc Fragment Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): Bovine,Equine,Human

Product Properties

• Clonality: Polyclonal
• Concentration: 1.20 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:450,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:50,000
• Application Notes: Anti-Mouse IgG F(c) antibody has been tested by ELISA and is suitable for ELISA, Western Blot and Immunohistochemistry applications. Antibody should be optimized by end user for specific reactive conditions.

Images

ELISA Results of purified Goat Anti-Mouse IgG F(c) Min X Bovine Horse and Human Serums. Each well was coated in duplicate with 1.0 µg of Mouse IgG (010-0102). The working dilution is 27300. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Dky Anti- Goat IgG HRP conjugated (p/n 605-703-125) and TMB substrate (p/n TMBE-1000).