Rabbit IgG anti-Mouse IgG (H+L)-HRPO, MinX Hu, Polyclonal

Produktübersicht

• Artikelname: Rabbit IgG anti-Mouse IgG (H+L)-HRPO, MinX Hu, Polyclonal
• Artikelnummer: ROC-610-4320
• Hersteller Artikelnummer: 610-4320
• Alternativnummer: ROC-610-4320
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgG whole molecule
• Konjugation: HRPO
• Alternative Synonym: Rabbit Anti-Mouse IgG Antibody peroxidase Conjugation, Rabbit Anti-Mouse IgG Antibody peroxidase Conjugated, Rabbit Anti-Mouse IgG HRP Conjugated Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): Human

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:150,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry. Specific conditions for reactivity and signal detection should be optimized

Bilder

ELISA results of purified Rabbit Anti-Mouse IgG Peroxidase Conjugated Antibody tested against purified Mouse IgG. (Red Line). Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000) and TMB substrate (p/n TMBE-1000).