Rabbit IgG anti-Mouse IgG (H+L)-HRPO, MinX Hu, Polyclonal

Product Overview

• Article Name: Rabbit IgG anti-Mouse IgG (H+L)-HRPO, MinX Hu, Polyclonal
• Biozol Catalog Number: ROC-610-4320
• Supplier Catalog Number: 610-4320
• Alternative Catalog Number: ROC-610-4320
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG whole molecule
• Conjugation: HRPO
• Alternative Names: Rabbit Anti-Mouse IgG Antibody peroxidase Conjugation, Rabbit Anti-Mouse IgG Antibody peroxidase Conjugated, Rabbit Anti-Mouse IgG HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Human

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:150,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry. Specific conditions for reactivity and signal detection should be optimized

Images

ELISA results of purified Rabbit Anti-Mouse IgG Peroxidase Conjugated Antibody tested against purified Mouse IgG. (Red Line). Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000) and TMB substrate (p/n TMBE-1000).