Sheep IgG anti-Mouse IgG (H+L)-unconj., MinX none

Produktübersicht

• Artikelname: Sheep IgG anti-Mouse IgG (H+L)-unconj., MinX none
• Artikelnummer: ROC-610-601-002
• Hersteller Artikelnummer: 610-601-002
• Alternativnummer: ROC-610-601-002
• Hersteller: Rockland Immunochemicals
• Wirt: Sheep
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Mouse
• Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in sheep.
• Konjugation: Unconjugated
• Alternative Synonym: sheep anti-Mouse IgG Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Mouse IgG antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by

Bilder

ELISA results of purified Sheep anti-Mouse IgG tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 010-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Dky-anti-Sheep HRP conjugate (p/n 613-7302), and TMB substrate (p/n TMBE-1000).