Sheep IgG anti-Mouse IgG (H+L)-unconj., MinX none

Product Overview

• Article Name: Sheep IgG anti-Mouse IgG (H+L)-unconj., MinX none
• Biozol Catalog Number: ROC-610-601-002
• Supplier Catalog Number: 610-601-002
• Alternative Catalog Number: ROC-610-601-002
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in sheep.
• Conjugation: Unconjugated
• Alternative Names: sheep anti-Mouse IgG Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Mouse IgG antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by

Images

ELISA results of purified Sheep anti-Mouse IgG tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 010-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Dky-anti-Sheep HRP conjugate (p/n 613-7302), and TMB substrate (p/n TMBE-1000).