Sheep IgG anti-Mouse IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Produktübersicht

• Artikelname: Sheep IgG anti-Mouse IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Artikelnummer: ROC-610-605-002
• Hersteller Artikelnummer: 610-605-002
• Alternativnummer: ROC-610-605-002
• Hersteller: Rockland Immunochemicals
• Wirt: Sheep
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgG whole molecule
• Konjugation: Alk. Phos.
• Alternative Synonym: sheep anti-Mouse IgG Alkaline Phosphatase Conjugated Antibody, sheep anti-Mouse IgG Antibody Alkaline Phosphatase Conjugation
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: Anti-Mouse IgG Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature.

Bilder

Dot Blot of Sheep anti-Mouse IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Sheep anti-Mouse IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Reaction visualized using alkaline phosphatase substrate (NBT-100) for 30 seconds at RT.