Sheep IgG anti-Mouse IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Sheep IgG anti-Mouse IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-610-605-002
• Supplier Catalog Number: 610-605-002
• Alternative Catalog Number: ROC-610-605-002
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG whole molecule
• Conjugation: Alk. Phos.
• Alternative Names: sheep anti-Mouse IgG Alkaline Phosphatase Conjugated Antibody, sheep anti-Mouse IgG Antibody Alkaline Phosphatase Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Mouse IgG Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature.

Images

Dot Blot of Sheep anti-Mouse IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Sheep anti-Mouse IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Reaction visualized using alkaline phosphatase substrate (NBT-100) for 30 seconds at RT.