Goat IgG anti-Rabbit IgG (F(ab)2)-unconj., MinX none

Produktübersicht

• Artikelname: Goat IgG anti-Rabbit IgG (F(ab)2)-unconj., MinX none
• Artikelnummer: ROC-611-1104
• Hersteller Artikelnummer: 611-1104
• Alternativnummer: ROC-611-1104
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Rabbit
• Immunogen: Rabbit IgG F(ab)2 fragment
• Konjugation: Unconjugated
• Alternative Synonym: goat anti Rabbit IgG F(ab)2 Antibody, goat anti Rabbit IgG Fab2 Antibody, goat anti-Rabbit IgG F(ab)2 Antibody, goat anti-Rabbit IgG Fab2 Antibody
• Format: IgG
• Spezifität: IgG (F(ab')2)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 10.2mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Rabbit
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:30,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000
• Anwendungsbeschreibung: Anti-Rabbit IgG F(ab)2 antibody has been tested by ELISA, dot blot, and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Bilder

Western Blot of Anti-Rabbit IgG F(ab)2 (GOAT) Antibody (p/n 611-1104). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(ab)2 (GOAT) Antibody (p/n 611-1104) 1:10,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.