Goat IgG anti-Rabbit IgG (F(ab)2)-unconj., MinX none

Product Overview

• Article Name: Goat IgG anti-Rabbit IgG (F(ab)2)-unconj., MinX none
• Biozol Catalog Number: ROC-611-1104
• Supplier Catalog Number: 611-1104
• Alternative Catalog Number: ROC-611-1104
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rabbit
• Immunogen: Rabbit IgG F(ab)2 fragment
• Conjugation: Unconjugated
• Alternative Names: goat anti Rabbit IgG F(ab)2 Antibody, goat anti Rabbit IgG Fab2 Antibody, goat anti-Rabbit IgG F(ab)2 Antibody, goat anti-Rabbit IgG Fab2 Antibody
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 10.2mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:30,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000
• Application Notes: Anti-Rabbit IgG F(ab)2 antibody has been tested by ELISA, dot blot, and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of Anti-Rabbit IgG F(ab)2 (GOAT) Antibody (p/n 611-1104). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(ab)2 (GOAT) Antibody (p/n 611-1104) 1:10,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.