Rabbit IgG Fc Antibody Texas Red Conjugated, Donkey, Polyclonal

Produktübersicht

• Artikelname: Rabbit IgG Fc Antibody Texas Red Conjugated, Donkey, Polyclonal
• Artikelnummer: ROC-611-709-003
• Hersteller Artikelnummer: 611-709-003
• Alternativnummer: ROC-611-709-003
• Hersteller: Rockland Immunochemicals
• Wirt: Donkey
• Kategorie: Antikörper
• Applikation: DOT, WB
• Spezies Reaktivität: Rabbit
• Immunogen: Rabbit IgG F(c) fragment
• Konjugation: Texas Red
• Alternative Synonym: Donkey Anti Rabbit IgG F(c) Texas Red(TM) Conjugated Antibody, Donkey Anti-Rabbit IgG Fc Fragment Antibody Texas Red(TM) conjugation, Donkey Anti Rabbit IgG Fc Antibody Texas Red(TM) conjugated

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rabbit
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: Flow Cytometry: User Optimized, IF Microscopy: 1:500 - 1:2,500
• Anwendungsbeschreibung: Anti-Rabbit IgG F(c) Texas Red antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex

Bilder

Western Blot of Anti-Rabbit IgG F(c) (DONKEY) Antibody (p/n 611-701-003). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (DONKEY) Antibody (p/n 611-701-003) 1:5,000 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.