Rabbit IgG Fc Antibody Texas Red Conjugated, Donkey, Polyclonal
Biozol Catalog Number:
ROC-611-709-003
Supplier Catalog Number:
611-709-003
Alternative Catalog Number:
ROC-611-709-003
Manufacturer:
Rockland Immunochemicals
Host:
Donkey
Category:
Antikörper
Application:
DOT, WB
Species Reactivity:
Rabbit
Immunogen:
Rabbit IgG F(c) fragment
Conjugation:
Texas Red
Alternative Names:
Donkey Anti Rabbit IgG F(c) Texas Red(TM) Conjugated Antibody, Donkey Anti-Rabbit IgG Fc Fragment Antibody Texas Red(TM) conjugation, Donkey Anti Rabbit IgG Fc Antibody Texas Red(TM) conjugated
Clonality:
Polyclonal
Concentration:
2.0 mg/mL by UV absorbance at 280 nm
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Rabbit
Antibody Type:
Secondary Antibody
Application Dilute:
Flow Cytometry: User Optimized, IF Microscopy: 1:500 - 1:2,500
Application Notes:
Anti-Rabbit IgG F(c) Texas Red antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex
Western Blot of Anti-Rabbit IgG F(c) (DONKEY) Antibody (p/n 611-701-003). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (DONKEY) Antibody (p/n 611-701-003) 1:5,000 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
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