Goat IgG anti-Rat IgM (µ)-HRPO, MinX none

Produktübersicht

• Artikelname: Goat IgG anti-Rat IgM (µ)-HRPO, MinX none
• Artikelnummer: ROC-612-1307
• Hersteller Artikelnummer: 612-1307
• Alternativnummer: ROC-612-1307
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Rat
• Immunogen: Rat IgM whole molecule
• Konjugation: HRPO
• Alternative Synonym: goat anti-Rat IgM (mu chain) Antibody peroxidase Conjugation, goat anti-Rat IgM mu chain HRP Conjugated Antibody
• Format: IgG
• Spezifität: IgM (µ)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rat
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:90,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Rat IgM Peroxidase Conjugated antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Bilder

Western Blot of Anti-Rat IgM (mu chain) (GOAT) Antibody (p/n 612-1107). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody (p/n 612-1107) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.