Goat IgG anti-Rat IgM (µ)-HRPO, MinX none

Product Overview

• Article Name: Goat IgG anti-Rat IgM (µ)-HRPO, MinX none
• Biozol Catalog Number: ROC-612-1307
• Supplier Catalog Number: 612-1307
• Alternative Catalog Number: ROC-612-1307
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rat
• Immunogen: Rat IgM whole molecule
• Conjugation: HRPO
• Alternative Names: goat anti-Rat IgM (mu chain) Antibody peroxidase Conjugation, goat anti-Rat IgM mu chain HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgM (µ)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:90,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Rat IgM Peroxidase Conjugated antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of Anti-Rat IgM (mu chain) (GOAT) Antibody (p/n 612-1107). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody (p/n 612-1107) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.