Aquaporin 2 phospho S261 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Aquaporin 2 phospho S261 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-612-401-D08
• Hersteller Artikelnummer: 612-401-D08
• Alternativnummer: ROC-612-401-D08
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IHC, IP, WB
• Spezies Reaktivität: Mouse, Rat
• Immunogen: Anti-Aquaporin 2 pS261 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser261.
• Konjugation: Unconjugated
• Alternative Synonym: ADH water channel, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 25386
• UniProt: P34080
• Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Formulierung: Liquid
• Target-Kategorie: Rat
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000, WB: 1:1000
• Anwendungsbeschreibung: Anti-Aquaporin 2 pS261 antibody is tested for use in ICC, IHC, IP, and WB. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa AQP2 protein phosphorylated at Ser261 in the appropriate cell lysate

Bilder

Western Blot of Rabbit anti-Aquaporin 2 pS261 antibody. Lane 1: rat kidney lysate. Lane 2: rat kidney lysate blocked by the phospho-peptide. Load: 10 µg per lane. Primary antibody: Aquaporin 2 pS261 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~ 29kDa and 37kDa glycosylated form of the AQP2 protein phosphorylated at Ser261. Other band(s): AQP2 splice variants and isoforms.