Anti-Aquaporin 2 pS261 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser261.
Conjugation:
Unconjugated
Alternative Names:
ADH water channel, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct
Clonality:
Polyclonal
Concentration:
Titrated value sufficient to run approximately 10 mini blots.
Anti-Aquaporin 2 pS261 antibody is tested for use in ICC, IHC, IP, and WB. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa AQP2 protein phosphorylated at Ser261 in the appropriate cell lysate
Western Blot of Rabbit anti-Aquaporin 2 pS261 antibody. Lane 1: rat kidney lysate. Lane 2: rat kidney lysate blocked by the phospho-peptide. Load: 10 µg per lane. Primary antibody: Aquaporin 2 pS261 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~ 29kDa and 37kDa glycosylated form of the AQP2 protein phosphorylated at Ser261. Other band(s): AQP2 splice variants and isoforms.
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