MEK5 phospho S311/phospho T315 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: MEK5 phospho S311/phospho T315 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-612-401-D74
• Hersteller Artikelnummer: 612-401-D74
• Alternativnummer: ROC-612-401-D74
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Rat
• Immunogen: Anti-MEK5 pS311/pT315 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 conjugated to KLH.
• Konjugation: Unconjugated
• Alternative Synonym: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 29568
• UniProt: Q62862
• Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Formulierung: Liquid
• Target-Kategorie: Rat
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB: 1:1000
• Anwendungsbeschreibung: Anti-MEK5 pS311/pT315 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 49 kDa in size corresponding to MEK5 protein phosphorylated at Ser311/Th

Bilder

Western Blot of Rabbit Anti-MEK5 pS311/pT315 antibody. Lane 1: rat testes. Lane 2: rat testes blocked by preadsorption with the phospho-peptide. Load: 10 µg per lane. Primary antibody: MEK5 pS311/pT315 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~49kDa/~49kDa for MEK5 protein. Other band(s): none.