MEK5 phospho S311/phospho T315 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: MEK5 phospho S311/phospho T315 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-612-401-D74
• Supplier Catalog Number: 612-401-D74
• Alternative Catalog Number: ROC-612-401-D74
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Rat
• Immunogen: Anti-MEK5 pS311/pT315 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5

Product Properties

• Clonality: Polyclonal
• Concentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 29568
• UniProt: Q62862
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: 1:1000
• Application Notes: Anti-MEK5 pS311/pT315 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 49 kDa in size corresponding to MEK5 protein phosphorylated at Ser311/Th

Images

Western Blot of Rabbit Anti-MEK5 pS311/pT315 antibody. Lane 1: rat testes. Lane 2: rat testes blocked by preadsorption with the phospho-peptide. Load: 10 µg per lane. Primary antibody: MEK5 pS311/pT315 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~49kDa/~49kDa for MEK5 protein. Other band(s): none.