NSF Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-612-401-E01
Artikelname: NSF Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-612-401-E01
Hersteller Artikelnummer: 612-401-E01
Alternativnummer: ROC-612-401-E01
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Rat
Immunogen: Anti-NSF Antibody was produced by repeated immunizations with synthetic peptide corresponding to amino acid residues from the C-terminal region.
Konjugation: Unconjugated
Alternative Synonym: Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF
Klonalität: Polyclonal
NCBI: 60355
UniProt: Q9QUL6
Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Formulierung: Liquid
Target-Kategorie: Rat
Antibody Type: Primary Antibody
Application Verdünnung: WB: 1:1000
Anwendungsbeschreibung: Anti-NSF Antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 75 kDa in size corresponding to the NSF protein in the appropriate cell lysate or extract.
Western Blot of Rabbit anti-NSF (N-ethylmaleimide sensitive fusion protein) antibody. Lane 1: rat caudate lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: NSF antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 75 kDa for NSF. Other band(s): none.