Anti-NSF Antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 75 kDa in size corresponding to the NSF protein in the appropriate cell lysate or extract.
Western Blot of Rabbit anti-NSF (N-ethylmaleimide sensitive fusion protein) antibody. Lane 1: rat caudate lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: NSF antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 75 kDa for NSF. Other band(s): none.
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